Akashi, Ok., Traver, D., Miyamoto, T. & Weissman, I. L. A clonogenic frequent myeloid progenitor that provides rise to all myeloid lineages. Nature 404, 193–197 (2000).
Klemsz, M. J., McKercher, S. R., Celada, A., Van Beveren, C. & Maki, R. A. The macrophage and B cell-specific transcription issue PU.1 is expounded to the ets oncogene. Cell 61, 113–124 (1990).
Reddy, M. A. et al. Opposing actions of c-ets/PU.1 and c-myb protooncogene merchandise in regulating the macrophage-specific promoters of the human and mouse colony-stimulating factor-1 receptor (c-fms) genes. J. Exp. Med. 180, 2309–2319 (1994).
Zhang, D. E., Hetherington, C. J., Chen, H. M. & Tenen, D. G. The macrophage transcription issue PU. 1 directs tissue-specific expression of the macrophage colony-stimulating issue receptor. Mol. Cell. Biol. 14, 373–373 (1994).
Hohaus, S. et al. PU. 1 (Spi-1) and C/EBP alpha regulate expression of the granulocyte-macrophage colony-stimulating issue receptor alpha gene. Mol. Cell. Biol. 15, 5830–5830 (1995).
Smith, L. T., Hohaus, S., Gonzalez, D. A., Dziennis, S. E. & Tenen, D. G. PU. 1 (Spi-1) and C/EBP alpha regulate the granulocyte colony-stimulating issue receptor promoter in myeloid cells. Blood 88, 1234–1234 (1996).
Iwasaki, H. et al. Distinctive and indispensable roles of PU. 1 in upkeep of hematopoietic stem cells and their differentiation. Blood 106, 1590–1590 (2005).
Staber, P. B. et al. Sustained PU.1 ranges steadiness cell-cycle regulators to stop exhaustion of grownup hematopoietic stem cells. Mol. Cell 49, 934–946 (2013).
Staber, P. B. et al. The Runx-PU.1 pathway preserves regular and AML/ETO9a leukemic stem cells. Blood 124, 2391–2399 (2014).
Rosenbauer, F. et al. Acute myeloid leukemia induced by graded discount of a lineage-specific transcription issue, PU.1. Nat. Genet. 36, 624–630 (2004).
Metcalf, D. et al. Inactivation of PU. 1 in grownup mice results in the event of myeloid leukemia. Proc. Natl Acad. Sci. USA 103, 1486–1486 (2006).
Burda, P. et al. PU. 1 activation relieves GATA-1–mediated repression of Cebpa and Cbfb throughout leukemia differentiation. Mol. Most cancers Res. 7, 1693–1693 (2009).
Liew, C. W. Molecular evaluation of the interplay between the hematopoietic grasp transcription elements GATA-1 and PU.1. J. Biol. Chem. 281, 28296–28306 (2006).
Nerlov, C., Querfurth, E., Kulessa, H. & Graf, T. GATA-1 interacts with the myeloid PU.1 transcription issue and represses PU.1-dependent transcription. Blood 95, 2543–2551 (2000).
Stopka, T., Amanatullah, D. F., Papetti, M. & Skoultchi, A. I. PU. 1 inhibits the erythroid program by binding to GATA-1 on DNA and making a repressive chromatin construction. EMBO J. 24, 3712–3712 (2005).
Takemoto, C. M. et al. PU.1 positively regulates GATA-1 expression in mast cells. J. Immunol. 184 4349–4361 (2010).
Zhang, P. et al. Unfavorable cross-talk between hematopoietic regulators: GATA proteins repress PU. 1. Proc. Natl Acad. Sci. USA 96, 8705–8705 (1999).
Zhang, P. et al. PU. 1 inhibits GATA-1 perform and erythroid differentiation by blocking GATA-1 DNA binding. Blood 96, 2641–2641 (2000).
Walsh, J. C. et al. Cooperative and antagonistic interaction between PU. 1 and GATA-2 within the specification of myeloid cell fates. Immunity 17, 665–676 (2002).
Chang, H. C. et al. PU. 1 regulates TCR expression by modulating GATA-3 exercise. J. Immunol. 183, 4887–4887 (2009).
Chang, H.-C. et al. PU.1 expression delineates heterogeneity in major Th2 cells. Immunity 22, 693–703 (2005).
Petrovick, M. S. et al. A number of useful domains of AML1: PU. 1 and C/EBPalpha synergize with totally different areas of AML1. Mol. Cell. Biol. 18, 3915–3915 (1998).
Reddy, V. A. et al. Granulocyte inducer C/EBPalpha inactivates the myeloid grasp regulator PU.1: potential position in lineage dedication selections. Blood 100, 483–490 (2002).
Yang, Z., Wara-Aswapati, N., Chen, C., Tsukada, J. & Auron, P. E. NF-IL6 (C/EBPbeta) vigorously prompts il1b gene expression through a Spi-1 (PU.1) protein-protein tether. J. Biol. Chem. 275, 21272–21277 (2000).
Ueki, N., Zhang, L. & Haymann, M. J. Ski can negatively regulates macrophage differentiation by way of its interplay with PU.1. Oncogene 27, 300–307 (2007).
Laricchia-Robbio, L., Premanand, Ok., Rinaldi, C. R. & Nucifora, G. EVI1 impairs myelopoiesis by deregulation of PU. 1 perform. Most cancers Res. 69, 1633–1633 (2009).
Rekhtman, N. et al. PU. 1 and pRB work together and cooperate to repress GATA-1 and block erythroid differentiation. Mol. Cell. Biol. 23, 7460–7460 (2003).
Wei, F., Zaprazna, Ok., Wang, J. & Atchison, M. L. PU.1 can recruit BCL6 to DNA to repress gene expression in germinal heart B cells. Mol. Cell. Biol. 29, 4612–4622 (2009).
Pongubala, J. M. et al. PU.1 recruits a second nuclear issue to a website vital for immunoglobulin kappa 3’ enhancer exercise. Mol. Cell. Biol. 12, 368–378 (1992).
Meraro, D. et al. Protein-protein and DNA-protein interactions have an effect on the exercise of lymphoid-specific IFN regulatory elements. J. Immunol. 163, 6468–6478 (1999).
Akashi, Ok. Lineage promiscuity and plasticity in hematopoietic improvement. Ann. N. Y. Acad. Sci. 1044, 125–131 (2005).
Rekhtman, N., Radparvar, F., Evans, T. & Skoultchi, A. I. Direct interplay of hematopoietic transcription elements PU.1 and GATA-1: useful antagonism in erythroid cells. Genes Dev. 13, 1398–1411 (1999).
Lord, Ok. A., Abdollahi, A., Hoffman-Liebermann, B. & Liebermann, D. A. Proto-oncogenes of the fos/jun household of transcription elements are optimistic regulators of myeloid differentiation. Mol. Cell. Biol. 13, 841–851 (1993).
Mechta-Grigoriou, F., Gerald, D. & Yaniv, M. The mammalian Jun proteins: redundancy and specificity. Oncogene 20, 2378–2389 (2001).
Jochum, W., Passegué, E. & Wagner, E. F. AP-1 in mouse improvement and tumorigenesis. Oncogene 20, 2401–2412 (2001).
Passegué, E., Wagner, E. F. & Weissman, I. L. JunB deficiency results in a myeloproliferative dysfunction arising from hematopoietic stem cells. Cell 119, 431–443 (2004).
Gaynor, R., Simon, Ok. & Koeffler, P. Expression of c-jun throughout macrophage differentiation of HL-60 cells. Blood 77, 2618–2623 (1991).
Hass, R., Prudovsky, I. & Kruhøffer, M. Differential results of phorbol ester on signaling and gene expression in human leukemia cells. Leuk. Res. 21, 589–594 (1997).
Sherman, M. L., Stone, R. M., Datta, R., Bernstein, S. H. & Kufe, D. W. Transcriptional and post-transcriptional regulation of c-jun expression throughout monocytic differentiation of human myeloid leukemic cells. J. Biol. Chem. 265, 3320–3323 (1990).
Grant, S. et al. Impact of 1-beta-D-arabinofuranosylcytosine on apoptosis and differentiation in human monocytic leukemia cells (U937) expressing a c-Jun dominant-negative mutant protein (TAM67). Cell Development Differ. 7, 603–613 (1996).
Behre, G. et al. c-Jun is a JNK-independent coactivator of the PU. 1 transcription issue. J. Biol. Chem. 274, 4939–4946 (1999).
Grondin, B. et al. c-Jun homodimers can perform as a context-specific coactivator. Mol. Cell. Biol. 27, 2919–2933 (2007).
Jia, X., Lee, L. Ok., Gentle, J., Palmer, A. G. & Assa-Munt, N. Spine dynamics of a brief PU.1 ETS area. J. Mol. Biol. 292, 1083–1093 (1999).
Kodandapani, R. et al. A brand new sample for helix-turn-helix recognition revealed by the PU.1 ETS-domain-DNA advanced. Nature 380, 456–460 (1996).
Bassuk, A. G. & Leiden, J. M. A direct bodily affiliation between ETS and AP-1 transcription elements in regular human T cells. Immunity 3, 223–237 (1995).
Vangala, R. Ok. et al. The myeloid grasp regulator transcription issue PU. 1 is inactivated by AML1-ETO in t (8; 21) myeloid leukemia. Blood 101, 270–270 (2003).
Uppaluri, R. & Towle, H. C. Genetic dissection of thyroid hormone receptor beta: identification of mutations that separate hormone binding and transcriptional activation. Mol. Cell. Biol. 15, 1499–1512 (1995).
Shih, H. M. et al. A optimistic genetic choice for disrupting protein-protein interactions: identification of CREB mutations that stop affiliation with the coactivator CBP. Proc. Natl Acad. Sci. USA 93, 13896–13901 (1996).
Escalante, C. R. et al. Crystallization and characterization of PU.1/IRF-4/DNA ternary advanced. J. Struct. Biol. 139, 55–59 (2002).
McKercher, S. R., Lombardo, C. R., Bobkov, A., Jia, X. & Assa-Munt, N. Identification of a PU. 1–IRF4 protein interplay floor predicted by chemical alternate line broadening. Proc. Natl Acad. Sci. USA 100, 511–511 (2003).
Szabo, E., Preis, L. H. & Birrer, M. J. Constitutive cJun expression induces partial macrophage differentiation in U-937 cells. Cell Development Differ. 5, 439–446 (1994).
Li, J., King, I. & Sartorelli, A. C. Differentiation of WEHI-3B D+ myelomonocytic leukemia cells induced by ectopic expression of the protooncogene c-jun. Cell Development Differ. 5, 743–751 (1994).
Wang, X. & Studzinski, G. P. The requirement for and altering composition of the activating protein-1 transcription issue throughout differentiation of human leukemia HL60 cells induced by 1,25-dihydroxyvitamin D3. Most cancers Res. 66, 4402–4409 (2006).
Anderson, Ok. L., Smith, Ok. A., Pio, F., Torbett, B. E. & Maki, R. A. Neutrophils poor in PU. 1 don’t terminally differentiate or turn out to be functionally competent. Blood 92, 1576–1576 (1998).
Pear, W. S. et al. Environment friendly and fast induction of a persistent myelogenous leukemia-like myeloproliferative illness in mice receiving P210 bcr/abl-transduced bone marrow. Blood 92, 3780–3792 (1998).
Weigelt, Ok., Lichtinger, M., Rehli, M. & Langmann, T. Transcriptomic profiling identifies a PU.1 regulatory community in macrophages. Biochem. Biophys. Res. Commun. 380, 308–312 (2009).
Otero, Ok. et al. Macrophage colony-stimulating issue induces the proliferation and survival of macrophages through a pathway involving DAP12 and beta-catenin. Nat. Immunol. 10, 734–743 (2009).
Heinz, S. et al. Easy combos of lineage-determining transcription elements prime cis-regulatory components required for macrophage and B cell identities. Mol. Cell 38, 576–589 (2010).
Zambelli, F., Pesole, G. & Pavesi, G. Pscan: discovering over-represented transcription issue binding website motifs in sequences from co-regulated or co-expressed genes. Nucleic Acids Res. 37, W247–W252 (2009).
Portales-Casamar, E. et al. JASPAR 2010: the vastly expanded open-access database of transcription issue binding profiles. Nucleic Acids Res. 38, D105–D110 (2010).
Badis, G. et al. Variety and complexity in DNA recognition by transcription elements. Science 324, 1720–1723 (2009).
Gangenahalli, G. U. et al. Stem cell destiny specification: position of grasp regulatory swap transcription issue PU. 1 in differential hematopoiesis. Stem Cells Dev. 14, 140–152 (2005).
Tenen, D. G., Hromas, R., Licht, J. D. & Zhang, D. E. Transcription elements, regular myeloid improvement, and leukemia. Blood 90, 489–519 (1997).
Spooner, C. J., Cheng, J. X., Pujadas, E., Laslo, P. & Singh, H. A recurrent community involving the transcription elements PU.1 and Gfi1 orchestrates innate and adaptive immune cell fates. Immunity 31, 576–586 (2009).
Steidl, U. et al. Important position of Jun household transcription elements in PU.1 knockdown–induced leukemic stem cells. Nat. Genet. 38, 1269–1277 (2006).
Adolfsson, J. et al. Identification of Flt3+ lympho-myeloid stem cells missing erythro-megakaryocytic potentiala revised street map for grownup blood lineage dedication. Cell 121, 295–306 (2005).
Lavau, C., Szilvassy, S. J., Slany, R. & Cleary, M. L. Immortalization and leukemic transformation of a myelomonocytic precursor by retrovirally transduced HRX-ENL. EMBO J. 16, 4226–4237 (1997).
Higuchi, M. et al. Expression of a conditional AML1-ETO oncogene bypasses embryonic lethality and establishes a murine mannequin of human t(8;21) acute myeloid leukemia. Most cancers Cell 1, 63–74 (2002).
Huntly, B. J. et al. MOZ-TIF2, however not BCR-ABL, confers properties of leukemic stem cells to dedicated murine hematopoietic progenitors. Most cancers Cell 6, 587–596 (2004).
Pahl, H. L. et al. The proto-oncogene PU.1 regulates expression of the myeloid-specific CD11b promoter. J. Biol. Chem. 268, 5014–5020 (1993).
Behre, G., Smith, L. T. & Tenen, D. G. Use of a promoterless Renilla luciferase vector as an inner management plasmid for transient co-transfection assays of Ras-mediated transcription activation. BioTechniques 26, 24–26, 28-24-26, 28 (1999).
DeKoter, R. P., Lee, H.-J. & Singh, H. PU.1 regulates expression of the interleukin-7 receptor in lymphoid progenitors. Immunity 16, 297–309 (2002).
Tusher, V. G., Tibshirani, R. & Chu, G. Significance evaluation of microarrays utilized to the ionizing radiation response. Proc. Natl Acad. Sci. USA 98, 5116–5121 (2001).
Saeed, A. I. et al. TM4: a free, open-source system for microarray information administration and evaluation. BioTechniques 34, 374–378 (2003).
Schrodinger, LLC. The PyMOL Molecular Graphics System, Model 1.8 (2015).